› 8hk0

X-ray diffraction
2.29Å resolution

Crystal structure of Fic32-33 complex from Streptomyces ficellus NRRL 8067

Released:
DOI: 10.2210/pdb8hk0/pdb
PDB entry 8hk0 coloured by chain, front view.
PDB entry 8hk0 coloured by chain, side view.
PDB entry 8hk0 coloured by chain, top view.
Source organism: Streptomyces ficellus
Primary publication:
Oxidase Heterotetramer Completes 1-Azabicyclo[3.1.0]hexane Formation with the Association of a Nonribosomal Peptide Synthetase.
Cheng Y, Yi X, Zhang Y, He Q, Chen D, Cao W, Fang P, Liu W
J Am Chem Soc (2023)
PMID: 37043819

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215291 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acyl-CoA dehydrogenase/oxidase C-terminal domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 380 amino acids
Theoretical weight: 38.13 KDa
Source organism: Streptomyces ficellus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1W5T2G8 (Residues: 1-374; Coverage: 100%)
Gene name: EIZ62_06145
Sequence domains:
Acyl-CoA dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 384 amino acids
Theoretical weight: 41.2 KDa
Source organism: Streptomyces ficellus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1W5T3Z2 (Residues: 1-384; Coverage: 100%)
Gene name: EIZ62_06140
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL10U2
Spacegroup: P4212
Unit cell:
a: 137.08Å b: 137.08Å c: 148.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.226
Expression system: Escherichia coli

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