› 8hk5

Electron Microscopy
3Å resolution

C5aR1-Gi-C5a protein complex

Released:
DOI: 10.2210/pdb8hk5/pdb
PDB entry 8hk5 coloured by chain, front view.
PDB entry 8hk5 coloured by chain, side view.
PDB entry 8hk5 coloured by chain, top view.
Source organisms:
Primary publication:
Revealing the signaling of complement receptors C3aR and C5aR1 by anaphylatoxins.
Wang Y, Liu W, Xu Y, He X, Yuan Q, Luo P, Fan W, Zhu J, Zhang X, Cheng X, Jiang Y, Xu HE, Zhuang Y
Nat Chem Biol (2023)
PMID: 37169960
Related structures: EMD-34846

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-232734 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
C5a anaphylatoxin chemotactic receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 39.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21730 (Residues: 1-350; Coverage: 100%)
Gene names: C5AR, C5AR1, C5R1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
C5a anaphylatoxin Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P01031 (Residues: 678-751; Coverage: 5%)
Gene names: C5, CPAMD4
Sequence domains: Anaphylotoxin-like domain
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 353 amino acids
Theoretical weight: 40.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 2-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: D
Molecule details ›
Chain: D
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 67 amino acids
Theoretical weight: 7.43 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 2-68; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

1 bound ligand:
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-34846
Expression system: Spodoptera frugiperda

Quick links

Citations

1 citations in other articles

[Value of the expression levels of complement-3a receptor 1 and neutrophil extracellular traps in predicting sepsis-induced coagulopathy].
Cao et al. (2023)

1 mention without citation

Structural biology of complement receptors.
Santos-López et al. (2023)