› 8hkh

X-ray diffraction
2.7Å resolution

Crystal structure of the LC/A1-DARPin18 complex

Released:
DOI: 10.2210/pdb8hkh/pdb
PDB entry 8hkh coloured by chain, front view.
PDB entry 8hkh coloured by chain, side view.
PDB entry 8hkh coloured by chain, top view.
Source organisms:
Entry authors: Wu Y, Leka O, Kammerer R

Function and Biology Details

Reaction catalysed: 
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241897 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Botulinum neurotoxin A light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 49.95 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0DPI1 (Residues: 2-421; Coverage: 32%)
Gene names: CBO0806, CLC_0862, bna, botA
Sequence domains: Clostridial neurotoxin zinc protease
Designed ankyrin repeat protein 18 Chains: C, D
Molecule details ›
Chains: C, D
Length: 149 amino acids
Theoretical weight: 16.34 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 59.56Å b: 59.63Å c: 102.18Å
α: 74.98° β: 89.9° γ: 73.15°
R-values:
R R work R free
0.264 0.262 0.306
Expression system: Escherichia coli K-12

Quick links