› 8hkh

X-ray diffraction
2.7Å resolution

Crystal structure of the LC/A1-DARPin18 complex

Released:
DOI: 10.2210/pdb8hkh/pdb
PDB entry 8hkh coloured by chain, front view.
PDB entry 8hkh coloured by chain, side view.
PDB entry 8hkh coloured by chain, top view.
Source organisms:
Primary publication:
A DARPin promotes faster onset of botulinum neurotoxin A1 action.
Leka O, Wu Y, Zanetti G, Furler S, Reinberg T, Marinho J, Schaefer JV, Plückthun A, Li X, Pirazzini M, Kammerer RA
Nat Commun 14 8317 (2023)
PMID: 38110403

Function and Biology Details

Reaction catalysed: 
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272768 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Botulinum neurotoxin A light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 49.95 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli K-12
Designed ankyrin repeat protein 18 Chains: C, D
Molecule details ›
Chains: C, D
Length: 149 amino acids
Theoretical weight: 16.34 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 59.56Å b: 59.63Å c: 102.18Å
α: 74.98° β: 89.9° γ: 73.15°
R-values:
R R work R free
0.264 0.262 0.306
Expression system: Escherichia coli K-12

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