› 8hko

X-ray diffraction
2.1Å resolution

Mutated human ADP-ribosyltransferase 2 (PARP2) catalytic domain bound to Rucaparib

Released:
DOI: 10.2210/pdb8hko/pdb
PDB entry 8hko coloured by chain, front view.
PDB entry 8hko coloured by chain, side view.
PDB entry 8hko coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wang XY, Zhou J, Xu BL

Function and Biology Details

Reaction catalysed: 
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-577402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGN5 (Residues: 230-581; Coverage: 60%)
Gene names: ADPRT2, ADPRTL2, PARP2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand RPB 2 x RPB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P21
Unit cell:
a: 39.761Å b: 86.286Å c: 96.047Å
α: 90° β: 90.39° γ: 90°
R-values:
R R work R free
0.166 0.164 0.211
Expression system: Escherichia coli

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