› 8hls

X-ray diffraction
2.04Å resolution

Crystal structure of the sialidase Am1547 from A.muciniphila

Released:
DOI: 10.2210/pdb8hls/pdb
PDB entry 8hls coloured by chain, front view.
PDB entry 8hls coloured by chain, side view.
PDB entry 8hls coloured by chain, top view.
Source organism: Akkermansia muciniphila ATCC BAA-835
Entry authors: Rui B, Xinyue T

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-502532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 601 amino acids
Theoretical weight: 67.31 KDa
Source organism: Akkermansia muciniphila ATCC BAA-835
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: B2ULI1 (Residues: 1-595; Coverage: 100%)
Gene name: Amuc_1547
Sequence domains: BNR repeat-like domain

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 72.16Å b: 56.771Å c: 145.932Å
α: 90° β: 94.01° γ: 90°
R-values:
R R work R free
0.181 0.18 0.223
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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