› 8hn2

X-ray diffraction
2.3Å resolution

Selenomethionine-labelled soluble domain of Rieske iron-sulfur protein from chlorobaculum tepidum

Released:
DOI: 10.2210/pdb8hn2/pdb
PDB entry 8hn2 coloured by chain, front view.
PDB entry 8hn2 coloured by chain, side view.
PDB entry 8hn2 coloured by chain, top view.
Source organism: Chlorobaculum tepidum
Primary publication:
Soluble domains of cytochrome c-556 and Rieske iron-sulfur protein from Chlorobaculum tepidum: Crystal structures and interaction analysis.
Kishimoto H, Azai C, Yamamoto T, Mutoh R, Nakaniwa T, Tanaka H, Miyanoiri Y, Kurisu G, Oh-Oka H
OpenAccess logo Curr Res Struct Biol 5 100101 (2023)
PMID: 37180033

Function and Biology Details

Reaction catalysed: 
Plastoquinol + 2 oxidized plastocyanin + 2 H(+)(Side 1) = plastoquinone + 2 reduced plastocyanin + 4 H(+)(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-574068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b6-f complex iron-sulfur subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 11.67 KDa
Source organism: Chlorobaculum tepidum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F722 (Residues: 77-181; Coverage: 58%)
Gene names: CT0302, petC
Sequence domains: Rieske [2Fe-2S] domain

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2
Unit cell:
a: 130.64Å b: 58.695Å c: 49.68Å
α: 90° β: 112.04° γ: 90°
R-values:
R R work R free
0.207 0.206 0.232
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

The synergy between the PscC subunits for electron transfer to the P840 special pair in Chlorobaculum tepidum.
Lyratzakis et al. (2024)

1 mention without citation

Soluble domains of cytochrome c-556 and Rieske iron-sulfur protein from Chlorobaculum tepidum: Crystal structures and interaction analysis.
Kishimoto et al. (2023)