› 8hne

X-ray diffraction
1.13Å resolution

Crystal structure of the ancestral GH19 chitinase Anc4

Released:
DOI: 10.2210/pdb8hne/pdb
PDB entry 8hne coloured by chain, front view.
PDB entry 8hne coloured by chain, side view.
PDB entry 8hne coloured by chain, top view.
Source organism: synthetic construct
Entry authors: Kozome D, Laurino P

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anc4, ancestral GH19 chitinase Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.05 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand 1PG 1 x 1PG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P212121
Unit cell:
a: 32.14Å b: 76.24Å c: 78.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.25
Expression system: Escherichia coli BL21(DE3)

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