› 8hnj

X-ray diffraction
2.03Å resolution

Domain-stabilized glutamine-binding protein

Released:
DOI: 10.2210/pdb8hnj/pdb
PDB entry 8hnj coloured by chain, front view.
PDB entry 8hnj coloured by chain, side view.
PDB entry 8hnj coloured by chain, top view.
Source organism: Escherichia coli 908519
Primary publication:
Domain-wise dissection of thermal stability enhancement in multidomain proteins.
Oh J, Durai P, Kannan P, Park J, Yeon YJ, Lee WK, Park K, Seo MH
Int J Biol Macromol 237 124141 (2023)
PMID: 36958447

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine-binding periplasmic protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 230 amino acids
Theoretical weight: 25.54 KDa
Source organism: Escherichia coli 908519
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEQ3 (Residues: 27-246; Coverage: 97%)
Gene names: JW0796, b0811, glnH
Sequence domains: Bacterial extracellular solute-binding proteins, family 3

Ligands and Environments

1 bound ligand:
Ligand GLN 6 x GLN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 235.1Å b: 69.59Å c: 83.74Å
α: 90° β: 90.711° γ: 90°
R-values:
R R work R free
0.228 0.226 0.264
Expression system: Escherichia coli BL21(DE3)

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