› 8hoa

X-ray diffraction
1.68Å resolution

ScRIPK mutant K124R

Released:
DOI: 10.2210/pdb8hoa/pdb
PDB entry 8hoa coloured by chain, front view.
PDB entry 8hoa coloured by chain, side view.
PDB entry 8hoa coloured by chain, top view.
Source organism: Saccharum hybrid cultivar
Primary publication:
Receptor-like cytoplasmic kinase ScRIPK in sugarcane regulates disease resistance and drought tolerance in Arabidopsis.
Fang J, Chai Z, Huang R, Huang C, Ming Z, Chen B, Yao W, Zhang M
OpenAccess logo Front Plant Sci 14 1191449 (2023)
PMID: 37304725

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.2 KDa
Source organism: Saccharum hybrid cultivar
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A811MC32 (Residues: 85-395; Coverage: 71%)
Gene name: NCGR_LOCUS634
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand HX2 1 x HX2
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 42.66Å b: 82.35Å c: 98.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.213
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Emerging Roles of Receptor-like Protein Kinases in Plant Response to Abiotic Stresses.
Gandhi et al. (2023)

1 mention without citation

Receptor-like cytoplasmic kinase ScRIPK in sugarcane regulates disease resistance and drought tolerance in Arabidopsis.
Fang et al. (2023)