8hpa

Electron Microscopy
3.01Å resolution

Monkeypox virus DNA replication holoenzyme F8, A22 and E4 complex in a DNA binding form

Released:
Source organism: Monkeypox virus
Related structures: EMD-34929

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-269547 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 1029 amino acids
Theoretical weight: 120.04 KDa
Source organism: Monkeypox virus
Expression system: Insect expression vector pBlueBachsGCA1
UniProt:
  • Canonical: A0A7H0DN44 (Residues: 1-1006; Coverage: 100%)
Gene names: MPXVgp056, OPG071, POL
Sequence domains:
DNA polymerase processivity factor component OPG148 Chain: C
Molecule details ›
Chain: C
Length: 426 amino acids
Theoretical weight: 49.2 KDa
Source organism: Monkeypox virus
Expression system: Insect expression vector pBlueBachsGCA1
UniProt:
  • Canonical: A0A7H0DNC0 (Residues: 1-426; Coverage: 100%)
Gene names: MPXVgp132, OPG148
Sequence domains: Chordopoxvirus A20R protein
Uracil-DNA glycosylase Chain: B
DNA (5'-D(P*AP*TP*GP*GP*TP*AP*GP*GP*GP*GP*AP*AP*GP*GP*AP*TP*CP*G)-3') Chain: E
DNA (5'-D(*CP*GP*AP*TP*CP*CP*TP*TP*CP*CP*CP*CP*TP*AP*C)-3') Chain: D

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.01Å
Relevant EMDB volumes: EMD-34929
Expression system: Insect expression vector pBlueBachsGCA1