› 8hpb

Solution NMR

NMR Structure of OsCIE1-Ubox S237D mutant

Released:
DOI: 10.2210/pdb8hpb/pdb
PDB entry 8hpb coloured by chain, ensemble of 20 models, front view.
PDB entry 8hpb coloured by chain, ensemble of 20 models, side view.
PDB entry 8hpb coloured by chain, ensemble of 20 models, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Release of a ubiquitin brake activates OsCERK1-triggered immunity in rice.
Wang G, Chen X, Yu C, Shi X, Lan W, Gao C, Yang J, Dai H, Zhang X, Zhang H, Zhao B, Xie Q, Yu N, He Z, Zhang Y, Wang E
Nature (2024)
PMID: 38750355

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U-box domain-containing protein 12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 80 amino acids
Theoretical weight: 8.98 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5VRH9 (Residues: 227-303; Coverage: 13%)
Gene names: LOC_Os06g01304, OSJNBa0075G19.19-1, Os06g0102700, PUB12
Sequence domains: U-box domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 41%
Refinement method: simulated annealing
Expression system: Escherichia coli

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