› 8hpe

X-ray diffraction
3.22Å resolution

Crystal structure of Leucine dehydrogenase

Released:
DOI: 10.2210/pdb8hpe/pdb
PDB entry 8hpe coloured by chain, front view.
PDB entry 8hpe coloured by chain, side view.
PDB entry 8hpe coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
A Tri-Enzyme Cascade for Efficient Production of L-2-Aminobutyrate from L-Threonine.
Li X, Gao C, Wei W, Song W, Meng W, Liu J, Chen X, Gao C, Guo L, Liu L, Wu J
Chembiochem 24 e202300148 (2023)
PMID: 36946691

Function and Biology Details

Reaction catalysed: 
L-leucine + H(2)O + NAD(+) = 4-methyl-2-oxopentanoate + NH(3) + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 39.87 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A9X5N1L9 (Residues: 1-366; Coverage: 100%)
Gene names: BK774_17255, BK775_32270, BTGOE4_46100, BUM91_06765, D7J84_18335, F8517_09190, ldh1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P4212
Unit cell:
a: 120.949Å b: 120.949Å c: 177.624Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.295
Expression system: Escherichia coli

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