› 8hrx

Electron Microscopy
2.89Å resolution

Cryo-EM structure of human NTCP-myr-preS1-YN9048Fab complex

Released:
DOI: 10.2210/pdb8hrx/pdb
PDB entry 8hrx coloured by chain, front view.
PDB entry 8hrx coloured by chain, side view.
PDB entry 8hrx coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of hepatitis B virus receptor binding.
Asami J, Park JH, Nomura Y, Kobayashi C, Mifune J, Ishimoto N, Uemura T, Liu K, Sato Y, Zhang Z, Muramatsu M, Wakita T, Drew D, Iwata S, Shimizu T, Watashi K, Park SY, Nomura N, Ohto U
Nat Struct Mol Biol (2024)
PMID: 38233573
Related structures: EMD-34981

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-238978 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hepatic sodium/bile acid cotransporter Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q14973 (Residues: 1-319; Coverage: 91%)
Gene names: GIG29, NTCP, SLC10A1
Sequence domains: Sodium Bile acid symporter family
PreS1 protein Chain: B
Molecule details ›
Chain: B
Length: 55 amino acids
Theoretical weight: 6.23 KDa
Source organism: Hepatitis B virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6RXR5 (Residues: 2-48; Coverage: 37%)
Sequence domains: Major surface antigen from hepadnavirus
Fab heavy chain from antibody IgG clone number YN9048 Chain: H
Molecule details ›
Chain: H
Length: 246 amino acids
Theoretical weight: 26.41 KDa
Source organism: Ondatra zibethicus
Expression system: Mus musculus
Fab light chain from antibody IgG clone number YN9048 Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.83 KDa
Source organism: Ondatra zibethicus
Expression system: Mus musculus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.89Å
Relevant EMDB volumes: EMD-34981
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli
  • Mus musculus

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