› 8htw

X-ray diffraction
2Å resolution

Crystal Structure of the ring nuclease Sso2081 Y133F mutant from Saccharolobus solfataricus in its apo form

Released:
DOI: 10.2210/pdb8htw/pdb
PDB entry 8htw coloured by chain, front view.
PDB entry 8htw coloured by chain, side view.
PDB entry 8htw coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Molecular basis of stepwise cyclic tetra-adenylate cleavage by the type III CRISPR ring nuclease Crn1/Sso2081.
Du L, Zhang D, Luo Z, Lin Z
OpenAccess logo Nucleic Acids Res (2023)
PMID: 36807980

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system ring nuclease SSO2081 Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 20.54 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7LYJ6 (Residues: 1-178; Coverage: 100%)
Gene name: SSO2081
Sequence domains: CRISPR-associated protein NE0113 (Cas_NE0113)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 43.747Å b: 95.253Å c: 45.836Å
α: 90° β: 107.803° γ: 90°
R-values:
R R work R free
0.226 0.224 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Molecular mechanism of allosteric activation of the CRISPR ribonuclease Csm6 by cyclic tetra-adenylate.
Du et al. (2024)

1 mention without citation

Molecular basis of stepwise cyclic tetra-adenylate cleavage by the type III CRISPR ring nuclease Crn1/Sso2081.
Du et al. (2023)