› 8huf

X-ray diffraction
2.29Å resolution

B28 in complex with CRM1-Ran-RanBP1

Released:
DOI: 10.2210/pdb8huf/pdb
Model geometry
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PDB entry 8huf coloured by chain, front view.
PDB entry 8huf coloured by chain, side view.
PDB entry 8huf coloured by chain, top view.
Source organisms:
Primary publication:
Discovery of a Hidden Pocket beneath the NES Groove by Novel Noncovalent CRM1 Inhibitors.
Li C, Zhang Q, Huang W, Huang L, Long Q, Lei Y, Jia D, Yang S, Yang Y, Zhang X, Sun Q
J Med Chem 66 17044-17058 (2023)
PMID: 38105606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-151811 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GTP-binding nuclear protein Ran Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Ran-specific GTPase-activating protein 1 Chain: B
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 16.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P41920 (Residues: 62-201; Coverage: 70%)
Gene names: CST20, HTN1, SFO1, YD8119.08, YDR002W, YRB1
Sequence domains: RanBP1 domain
Exportin-1 Chain: C

Ligands and Environments

7 bound ligands:
Ligand MG 1 x MG
Ligand GOL 3 x GOL
Ligand GTP 1 x GTP
Ligand BR 1 x BR
Ligand CL 1 x CL
Ligand NA 1 x NA
Ligand N5X 1 x N5X
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P43212
Unit cell:
a: 106.361Å b: 106.361Å c: 304.641Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.222
Expression system: Escherichia coli

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