› 8hw4

Electron Microscopy
3.52Å resolution

Cryo-EM structure of dehydroepiandrosterone sulfate-bound human ABC transporter ABCC3 in nanodiscs

Released:
DOI: 10.2210/pdb8hw4/pdb
PDB entry 8hw4 coloured by chain, front view.
PDB entry 8hw4 coloured by chain, side view.
PDB entry 8hw4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Placing steroid hormones within the human ABCC3 transporter reveals a compatible amphiphilic substrate-binding pocket.
Wang J, Li X, Wang FF, Cheng MT, Mao YX, Fang SC, Wang L, Zhou CZ, Hou WT, Chen Y
EMBO J e113415 (2023)
PMID: 37485728
Related structures: EMD-35050

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
ATP + H(2)O + glutathione-S-conjugate(Side 1) = ADP + phosphate + glutathione-S-conjugate(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-binding cassette sub-family C member 3 Chain: A
Molecule details ›
Chain: A
Length: 1527 amino acids
Theoretical weight: 169.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O15438 (Residues: 1-1527; Coverage: 100%)
Gene names: ABCC3, CMOAT2, MLP2, MRP3
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZWY 2 x ZWY
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.52Å
Relevant EMDB volumes: EMD-35050
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Placing steroid hormones within the human ABCC3 transporter reveals a compatible amphiphilic substrate-binding pocket.
Wang et al. (2023)