› 8hx7

X-ray diffraction
1.95Å resolution

Crystal structure of 4-amino-4-deoxychorismate synthase from Streptomyces venezuelae co-crystallized with L-glutamine

Released:
DOI: 10.2210/pdb8hx7/pdb
PDB entry 8hx7 coloured by chain, front view.
PDB entry 8hx7 coloured by chain, side view.
PDB entry 8hx7 coloured by chain, top view.
Source organism: Streptomyces venezuelae
Primary publication:
Structural basis for the allosteric pathway of 4-amino-4-deoxychorismate synthase.
Nakamichi Y, Kobayashi J, Toyoda K, Suda M, Hiraga K, Inui M, Watanabe M
Acta Crystallogr D Struct Biol 79 895-908 (2023)
PMID: 37712435

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-514338 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxychorismate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 702 amino acids
Theoretical weight: 76.74 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2RB79 (Residues: 1-686; Coverage: 100%)
Gene names: SVEN_0920, cmlB, pabAB
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand TRP 2 x TRP
Ligand SIN 2 x SIN
Ligand MG 3 x MG
Ligand CL 1 x CL
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2221
Unit cell:
a: 98.291Å b: 170.915Å c: 160.344Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.24
Expression system: Escherichia coli BL21(DE3)

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