8i03

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the SIN3L complex from S. pombe

Released:
Source organism: Schizosaccharomyces pombe
Primary publication:
Two assembly modes for SIN3 histone deacetylase complexes.
OpenAccess logo Cell Discov 9 42 (2023)
PMID: 37076472
Related structures: EMD-35093

Function and Biology Details

Reaction catalysed:
Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-215113 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Paired amphipathic helix protein pst1 Chain: A
Molecule details ›
Chain: A
Length: 1522 amino acids
Theoretical weight: 171.67 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: Q09750 (Residues: 1-1522; Coverage: 100%)
Gene names: SPBC12C2.10c, SPBC21D10.01c, pst1
Sequence domains:
Paired amphipathic helix protein pst3 Chain: B
Molecule details ›
Chain: B
Length: 1154 amino acids
Theoretical weight: 133.05 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: O74755 (Residues: 1-1154; Coverage: 100%)
Gene names: SPBC1734.16c, pst3
Sequence domains:
Histone deacetylase clr6 Chains: C, D
Transcriptional regulatory protein dep1 Chain: E
Transcriptional regulatory protein rxt2 Chain: F
Transcriptional regulatory protein sds3 Chain: G
Chromatin modification-related protein png2 Chain: H
Transcriptional regulatory protein rxt3 Chain: I
RbAp48-related WD40 repeat-containing protein prw1 Chains: J, K

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-35093