› 8i3q

Electron Microscopy
3.1Å resolution

Cryo-EM structure of Cas12g-sgRNA binary complex

Released:
DOI: 10.2210/pdb8i3q/pdb
PDB entry 8i3q coloured by chain, front view.
PDB entry 8i3q coloured by chain, side view.
PDB entry 8i3q coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural transitions upon guide RNA binding and their importance in Cas12g-mediated RNA cleavage.
Liu M, Li Z, Chen J, Lin J, Lu Q, Ye Y, Zhang H, Zhang B, Ouyang S
OpenAccess logo PLoS Genet 19 e1010930 (2023)
PMID: 37729124
Related structures: EMD-35154

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240398 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cas12f1-like TNB domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 767 amino acids
Theoretical weight: 89.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A7C2YU39 (Residues: 1-767; Coverage: 100%)
Gene name: ENO60_02420
Sequence domains: Putative transposase DNA-binding domain
RNA (139-MER) Chain: C
Molecule details ›
Chain: C
Length: 139 nucleotides
Theoretical weight: 44.77 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-35154
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

A New RNA-Dependent Cas12g Nuclease.
Gunitseva et al. (2023)