› 8i4w

Electron Microscopy
6.01Å resolution

Cryo-EM structure of 5-subunit Smc5/6 head region

Released:
DOI: 10.2210/pdb8i4w/pdb
PDB entry 8i4w coloured by chain, front view.
PDB entry 8i4w coloured by chain, side view.
PDB entry 8i4w coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structures of Smc5/6 in multiple states reveal its assembly and functional mechanisms.
Qian L, Jun Z, Xiang Z, Wang Z, Tong C, Duo J, Zhenguo C, Wang L
Nat. Struct. Mol. Biol. (2024)
Related structures: EMD-35186

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-246368 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Structural maintenance of chromosomes protein 5 Chain: A
Molecule details ›
Chain: A
Length: 1093 amino acids
Theoretical weight: 126.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q08204 (Residues: 1-1093; Coverage: 100%)
Gene names: SMC5, YOL034W
Sequence domains: RecF/RecN/SMC N terminal domain
Structural maintenance of chromosomes protein 6 Chain: B
Molecule details ›
Chain: B
Length: 1114 amino acids
Theoretical weight: 128.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q12749 (Residues: 1-1114; Coverage: 100%)
Gene names: L3502.2, RHC18, SMC6, YLR383W
Sequence domains: AAA domain
DNA repair protein KRE29 Chain: D
Molecule details ›
Chain: D
Length: 464 amino acids
Theoretical weight: 53.84 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P40026 (Residues: 1-464; Coverage: 100%)
Gene names: KRE29, YER038C
Sequence domains: DNA repair proteins Nse5 and Nse6
Non-structural maintenance of chromosome element 5 Chain: E
Molecule details ›
Chain: E
Length: 556 amino acids
Theoretical weight: 64.08 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q03718 (Residues: 1-556; Coverage: 100%)
Gene names: NSE5, YML023C
Sequence domains: DNA repair proteins Nse5 and Nse6

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.01Å
Relevant EMDB volumes: EMD-35186
Expression system: Spodoptera frugiperda

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