› 8i54

Electron Microscopy
3.95Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-215094 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Lb2Cas12a Chain: A
Molecule details ›
Chain: A
Length: 1206 amino acids
Theoretical weight: 141.2 KDa
Source organism: Lachnospiraceae bacterium MA2020
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
DNA (25-MER) Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.78 KDa
Source organism: synthetic construct
Expression system: synthetic construct
DNA (5'-D(*AP*GP*TP*GP*CP*TP*TP*TP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 9 nucleotides
Theoretical weight: 2.75 KDa
Source organism: synthetic construct
Expression system: synthetic construct
RNA (33-MER) Chain: B
Molecule details ›
Chain: B
Length: 33 nucleotides
Theoretical weight: 10.45 KDa
Source organism: Lachnospiraceae bacterium MA2020
Expression system: synthetic construct

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.95Å
Relevant EMDB volumes: EMD-35191
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • synthetic construct

Quick links

Citations

1 review citation

Unity among the diverse RNA-guided CRISPR-Cas interference mechanisms.
Ganguly et al. (2024)
2 other articles

1 mention without citation

Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.
Jianwei et al. (2023)