› 8i58

X-ray diffraction
3.09Å resolution

Uroporphyrin I (UPI)-bound CfbA

Released:
DOI: 10.2210/pdb8i58/pdb
PDB entry 8i58 coloured by chain, front view.
PDB entry 8i58 coloured by chain, side view.
PDB entry 8i58 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Entry authors: Ogawa S, Fujishiro T

Function and Biology Details

Reactions catalysed: 
Ni-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Ni(2+)
Cobalt-precorrin-2 + 2 H(+) = precorrin-2 + Co(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sirohydrochlorin cobaltochelatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 16.55 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58380 (Residues: 1-143; Coverage: 100%)
Gene names: MJ0970, cbiX, cfbA
Sequence domains: CbiX

Ligands and Environments

1 bound ligand:
Ligand UPI 1 x UPI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P41
Unit cell:
a: 68.81Å b: 68.81Å c: 83.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.156 0.204
Expression system: Escherichia coli BL21(DE3)

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