› 8i5k

X-ray diffraction
1.22Å resolution

Crystal structure of chitin oligosaccharide binding protein from Vibrio cholera in complex with chitotriose.

Released:
DOI: 10.2210/pdb8i5k/pdb
PDB entry 8i5k coloured by chain, front view.
PDB entry 8i5k coloured by chain, side view.
PDB entry 8i5k coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Periplasmic chitooligosaccharide-binding protein requires a three-domain organization for substrate translocation.
Ohnuma T, Tsujii J, Kataoka C, Yoshimoto T, Takeshita D, Lampela O, Juffer AH, Suginta W, Fukamizo T
OpenAccess logo Sci Rep 13 20558 (2023)
PMID: 37996461

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-492661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Solute-binding protein family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 60.98 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A085SLP2 (Residues: 28-556; Coverage: 100%)
Gene names: D6U24_16190, ERS013201_01979, EYB64_10090, F0H40_11710, F0M16_04225, FLM12_17525, KIN13_14745, appA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 135.087Å b: 51.683Å c: 70.328Å
α: 90° β: 98.83° γ: 90°
R-values:
R R work R free
0.17 0.169 0.187
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Periplasmic chitooligosaccharide-binding protein requires a three-domain organization for substrate translocation.
Ohnuma et al. (2023)