› 8i80

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + viomycin = ADP + O-phosphoviomycin
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 30.44 KDa
Source organism: Streptosporangium roseum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D2B3F1 (Residues: 1-286; Coverage: 100%)
Gene name: Sros_4606
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

2 bound ligands:
Ligand PEG 5 x PEG
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P212121
Unit cell:
a: 82.299Å b: 86.937Å c: 87.015Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links