› 8i8j

X-ray diffraction
2.07Å resolution

Crystal structure of the ternary complex of Phosphopantetheine adenylyltransferase (PPAT) from Enterobacter sp. with Coenzyme-A and Phosphonoacetic acid at 2.07 A resolution.

Released:
DOI: 10.2210/pdb8i8j/pdb
PDB entry 8i8j coloured by chain, front view.
PDB entry 8i8j coloured by chain, side view.
PDB entry 8i8j coloured by chain, top view.
Source organism: Enterobacter sp. 638
Entry authors: Ahmad N, Sharma P, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed: 
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphopantetheine adenylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 166 amino acids
Theoretical weight: 18.44 KDa
Source organism: Enterobacter sp. 638
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4W515 (Residues: 1-159; Coverage: 100%)
Gene names: Ent638_0105, coaD
Sequence domains: Cytidylyltransferase-like

Ligands and Environments

4 bound ligands:
Ligand COA 2 x COA
Ligand PAE 6 x PAE
Ligand EDO 1 x EDO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 137.966Å b: 78.659Å c: 107.027Å
α: 90° β: 93.121° γ: 90°
R-values:
R R work R free
0.226 0.225 0.277
Expression system: Escherichia coli BL21(DE3)

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