› 8i8x

Electron Microscopy
3.25Å resolution

Cryo-EM Structure of OmpC3-MlaA-MlaC Complex in MSP2N2 Nanodiscs

Released:
DOI: 10.2210/pdb8i8x/pdb
PDB entry 8i8x coloured by chain, front view.
PDB entry 8i8x coloured by chain, side view.
PDB entry 8i8x coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular mechanism of phospholipid transport at the bacterial outer membrane interface.
Yeow J, Luo M, Chng SS
OpenAccess logo Nat Commun 14 8285 (2023)
PMID: 38092770
Related structures: EMD-35253

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-237000 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Outer membrane porin C Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 346 amino acids
Theoretical weight: 38.34 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06996 (Residues: 22-367; Coverage: 100%)
Gene names: JW2203, b2215, meoA, ompC, par
Sequence domains: Gram-negative porin
Intermembrane phospholipid transport system lipoprotein MlaA Chain: D
Molecule details ›
Chain: D
Length: 234 amino acids
Theoretical weight: 26.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P76506 (Residues: 18-251; Coverage: 100%)
Gene names: JW2343, b2346, mlaA, vacJ
Sequence domains: MlaA lipoprotein
Intermembrane phospholipid transport system binding protein MlaC Chain: F
Molecule details ›
Chain: F
Length: 199 amino acids
Theoretical weight: 22.9 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0ADV7 (Residues: 21-211; Coverage: 100%)
Gene names: JW3159, b3192, mlaC, yrbC
Sequence domains: MlaC protein

Ligands and Environments

1 bound ligand:
Ligand KDL 3 x KDL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.25Å
Relevant EMDB volumes: EMD-35253
Expression system: Escherichia coli BL21

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