› 8i95

Electron Microscopy
2.88Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-235042 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09471 (Residues: 4-57, 182-354; Coverage: 64%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Antibody fragment - ScFv16 Chain: H
Molecule details ›
Chain: H
Length: 248 amino acids
Theoretical weight: 26.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
C3a anaphylatoxin chemotactic receptor Chain: C
Molecule details ›
Chain: C
Length: 538 amino acids
Theoretical weight: 59.81 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16581 (Residues: 2-482; Coverage: 100%)
Gene names: AZ3B, C3AR1, C3R1, HNFAG09
Sequence domains: 7 transmembrane receptor (rhodopsin family)
EP54 ligand Chain: D
Molecule details ›
Chain: D
Length: 10 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
1 modified residue:
Ligand DAL 1 x DAL

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-35257
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

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