› 8i9l

Electron Microscopy
3.18Å resolution

Structure of C3a-C3aR-Go complex (Composite map)

Released:
DOI: 10.2210/pdb8i9l/pdb
PDB entry 8i9l coloured by chain, front view.
PDB entry 8i9l coloured by chain, side view.
PDB entry 8i9l coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of anaphylatoxin binding, activation, and signaling bias at complement receptors.
Yadav MK, Maharana J, Yadav R, Saha S, Sarma P, Soni C, Singh V, Saha S, Ganguly M, Li XX, Mohapatra S, Mishra S, Khant HA, Chami M, Woodruff TM, Banerjee R, Shukla AK, Gati C
OpenAccess logo Cell (2023)
PMID: 37852260
Related structures: EMD-35275

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235005 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09471 (Residues: 4-57, 182-354; Coverage: 64%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Antibody fragment - ScFv16 Chain: H
Molecule details ›
Chain: H
Length: 248 amino acids
Theoretical weight: 26.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
C3a anaphylatoxin chemotactic receptor Chain: C
Molecule details ›
Chain: C
Length: 538 amino acids
Theoretical weight: 59.81 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q16581 (Residues: 2-482; Coverage: 100%)
Gene names: AZ3B, C3AR1, C3R1, HNFAG09
Sequence domains: 7 transmembrane receptor (rhodopsin family)
C3a anaphylatoxin Chain: D
Molecule details ›
Chain: D
Length: 77 amino acids
Theoretical weight: 9.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01024 (Residues: 672-748; Coverage: 5%)
Gene names: C3, CPAMD1
Sequence domains: Anaphylotoxin-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.18Å
Relevant EMDB volumes: EMD-35275
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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