› 8ibs

X-ray diffraction
1.9Å resolution

Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E160A/E318A mutant in complex with galactose

Released:
DOI: 10.2210/pdb8ibs/pdb
PDB entry 8ibs coloured by chain, front view.
PDB entry 8ibs coloured by chain, side view.
PDB entry 8ibs coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Primary publication:
Substrate recognition mode of a glycoside hydrolase family 42 β-galactosidase from Bifidobacterium longum subspecies infantis (BiBga42A) revealed by crystallographic and mutational analyses.
Gotoh A, Hidaka M, Sakurama H, Nishimoto M, Kitaoka M, Sakanaka M, Fushinobu S, Katayama T
OpenAccess logo Microbiome Res Rep 2 20 (2023)
PMID: 38046823

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase III Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 702 amino acids
Theoretical weight: 78.69 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93GI5 (Residues: 1-691; Coverage: 100%)
Gene name: beta-galIII
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GLA 6 x GLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 234.47Å b: 182.691Å c: 143.263Å
α: 90° β: 125.73° γ: 90°
R-values:
R R work R free
0.189 0.187 0.228
Expression system: Escherichia coli BL21(DE3)

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