› 8id2

X-ray diffraction
1.8Å resolution

Crystal structure of the ubiquitin-like domain in the SF3A1 subunit of human U2 snRNP complexed with the stem-loop 4 of U1 snRNA

Released:
DOI: 10.2210/pdb8id2/pdb
Model geometry
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PDB entry 8id2 coloured by chain, front view.
PDB entry 8id2 coloured by chain, side view.
PDB entry 8id2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into recognition of SL4, the UUCG stem-loop, of human U1 snRNA by the ubiquitin-like domain, including the C-terminal tail in the SF3A1 subunit of U2 snRNP.
Nameki N, Terawaki SI, Takizawa M, Kitamura M, Muto Y, Kuwasako K
J Biochem (2023)
PMID: 37094335

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274565 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Splicing factor 3A subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 13.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15459 (Residues: 703-793; Coverage: 12%)
Gene names: SAP114, SF3A1
Sequence domains: Ubiquitin family
RNA (5'-R(*GP*GP*GP*GP*AP*CP*UP*GP*CP*GP*UP*UP*CP*GP*CP*GP*CP*UP*UP*UP*CP*CP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 24 nucleotides
Theoretical weight: 7.63 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 54.455Å b: 60.214Å c: 103.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

1 citations in other articles

Examining the capacity of human U1 snRNA variants to facilitate pre-mRNA splicing.
Wong et al. (2024)

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