8ifk

Electron Microscopy
2.54Å resolution

Cryo-EM structure of monomeric SPARTA gRNA-ssDNA target complex

Released:
Related structures: EMD-35419

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-238824 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
TIR domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 53.26 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A1I7NFG5 (Residues: 1-450; Coverage: 100%)
Gene name: SAMN05660895_1670
Sequence domains: TIR domain
Piwi domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 507 amino acids
Theoretical weight: 58.3 KDa
Source organism: Thermoflavifilum thermophilum
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A1I7NFD7 (Residues: 1-507; Coverage: 100%)
Gene name: SAMN05660895_1671
target ssDNA Chain: D
Molecule details ›
Chain: D
Length: 25 nucleotides
Theoretical weight: 7.68 KDa
guide RNA Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.66 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.54Å
Relevant EMDB volumes: EMD-35419
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'