› 8ifx

X-ray diffraction
2Å resolution

Aquifex aeolicus TsaD-TsaB in complex with ADP

Released:
DOI: 10.2210/pdb8ifx/pdb
PDB entry 8ifx coloured by chain, front view.
PDB entry 8ifx coloured by chain, side view.
PDB entry 8ifx coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Lu SZ, Zhang WH

Function and Biology Details

Reaction catalysed: 
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241869 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
tRNA N6-adenosine threonylcarbamoyltransferase Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 37.43 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66986 (Residues: 1-335; Coverage: 100%)
Gene names: aq_801, gcp, tsaD
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
Gcp-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 200 amino acids
Theoretical weight: 23.07 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66494 (Residues: 1-200; Coverage: 100%)
Gene name: aq_082
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase

Ligands and Environments

3 bound ligands:
Ligand FE 1 x FE
Ligand ADP 1 x ADP
Ligand PEG 3 x PEG
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-X
Spacegroup: P3121
Unit cell:
a: 98.017Å b: 98.017Å c: 131.432Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.253
Expression system: Escherichia coli

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