› 8ih7

X-ray diffraction
2.48Å resolution

AmnG-AmnH complex

Released:
DOI: 10.2210/pdb8ih7/pdb
PDB entry 8ih7 coloured by chain, front view.
PDB entry 8ih7 coloured by chain, side view.
PDB entry 8ih7 coloured by chain, top view.
Source organism: Pseudomonas sp.
Entry authors: Su D, Shi QL

Function and Biology Details

Reactions catalysed: 
(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate
Acetaldehyde + CoA + NAD(+) = acetyl-CoA + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275417 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
4-hydroxy-2-oxovalerate aldolase Chains: A, C
Molecule details ›
Chains: A, C
Length: 355 amino acids
Theoretical weight: 38.55 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KWS0 (Residues: 1-343; Coverage: 100%)
Gene name: amnG
Sequence domains:
Acetaldehyde dehydrogenase Chains: B, D
Molecule details ›
Chains: B, D
Length: 319 amino acids
Theoretical weight: 33.62 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWS1 (Residues: 1-316; Coverage: 100%)
Gene name: amnH
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand OXL 2 x OXL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 55.029Å b: 252.288Å c: 60.785Å
α: 90° β: 114.94° γ: 90°
R-values:
R R work R free
0.175 0.173 0.223
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

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