› 8ihg

X-ray diffraction
2.86Å resolution

Crystal structure of aminophenol dioxygenase from Pseudomonas species AP-3

Released:
DOI: 10.2210/pdb8ihg/pdb
PDB entry 8ihg coloured by chain, front view.
PDB entry 8ihg coloured by chain, side view.
PDB entry 8ihg coloured by chain, top view.
Source organism: Pseudomonas sp.
Entry authors: Shi QL, Su D

Function and Biology Details

Reaction catalysed: 
2-aminophenol + O(2) = 2-aminomuconate 6-semialdehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240467 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2-aminophenol 1,6-dioxygenase beta subunit Chains: A, C
Molecule details ›
Chains: A, C
Length: 305 amino acids
Theoretical weight: 34.48 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: O33477 (Residues: 1-305; Coverage: 100%)
Gene name: amnB
Sequence domains: Catalytic LigB subunit of aromatic ring-opening dioxygenase
2-aminophenol 1,6-dioxygenase alpha subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 272 amino acids
Theoretical weight: 29.46 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: O33478 (Residues: 2-271; Coverage: 100%)
Gene name: amnA
Sequence domains: Catalytic LigB subunit of aromatic ring-opening dioxygenase

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand 2AF 2 x 2AF
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P6122
Unit cell:
a: 153.475Å b: 153.475Å c: 312.036Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.234
Expression system: Escherichia coli

Quick links