› 8iiy

X-ray diffraction
2.15Å resolution

Crystal structure of MBP fused GAS41 YEATS domain in complex with H3K14ac peptide

Released:
DOI: 10.2210/pdb8iiy/pdb
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PDB entry 8iiy coloured by chain, front view.
PDB entry 8iiy coloured by chain, side view.
PDB entry 8iiy coloured by chain, top view.
Source organisms:
Primary publication:
GAS41 promotes H2A.Z deposition through recognition of the N terminus of histone H3 by the YEATS domain.
Kikuchi M, Takase S, Konuma T, Noritsugu K, Sekine S, Ikegami T, Ito A, Umehara T
Proc Natl Acad Sci U S A 120 e2304103120 (2023)
PMID: 37844223

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271927 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein; YEATS domain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 57.21 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: O95619 (Residues: 19-159; Coverage: 62%)
Gene names: GAS41, JW3994, YEATS4, b4034, malE
Sequence domains:
Histone H3.1 Chains: B, C
Molecule details ›
Chains: B, C
Length: 19 amino acids
Theoretical weight: 2.12 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-20; Coverage: 14%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P3221
Unit cell:
a: 66.241Å b: 66.241Å c: 248.821Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.17 0.247
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

1 citations in other articles

Targeting dependency on a paralog pair of CBP/p300 against de-repression of KREMEN2 in SMARCB1-deficient cancers.
Sasaki et al. (2024)

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