› 8ija

Electron Microscopy
2.69Å resolution

Cryo-EM structure of human HCAR2-Gi complex with niacin

Released:
DOI: 10.2210/pdb8ija/pdb
PDB entry 8ija coloured by chain, front view.
PDB entry 8ija coloured by chain, side view.
PDB entry 8ija coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into ligand recognition and selectivity of the human hydroxycarboxylic acid receptor HCAR2.
Pan X, Ye F, Ning P, Zhang Z, Li X, Zhang B, Wang Q, Chen G, Gao W, Qiu C, Wu Z, Li J, Zhu L, Xia J, Gong K, Du Y
Cell Discov 9 118 (2023)
PMID: 38012147
Related structures: EMD-35483

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-238156 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Hydroxycarboxylic acid receptor 2 Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 34.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TDS4 (Residues: 8-301; Coverage: 81%)
Gene names: GPR109A, HCA2, HCAR2, HM74A, NIACR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 351 amino acids
Theoretical weight: 40.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 4-354; Coverage: 99%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 337 amino acids
Theoretical weight: 37.07 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 4-340; Coverage: 99%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 56 amino acids
Theoretical weight: 6.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 8-63; Coverage: 79%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 248 amino acids
Theoretical weight: 26.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand NIO 1 x NIO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.69Å
Relevant EMDB volumes: EMD-35483
Expression system: Spodoptera frugiperda

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