› 8ikg

Electron Microscopy
3.4Å resolution

Cryo-EM structure of human receptor with G proteins

Released:
DOI: 10.2210/pdb8ikg/pdb
PDB entry 8ikg coloured by chain, front view.
PDB entry 8ikg coloured by chain, side view.
PDB entry 8ikg coloured by chain, top view.
Source organisms:
Primary publication:
Structure-based identification of a G protein-biased allosteric modulator of cannabinoid receptor CB1.
Shen S, Wu C, Lin G, Yang X, Zhou Y, Zhao C, Miao Z, Tian X, Wang K, Yang Z, Liu Z, Guo N, Li Y, Xia A, Zhou P, Liu J, Yan W, Ke B, Yang S, Shao Z
Proc Natl Acad Sci U S A 121 e2321532121 (2024)
PMID: 38830102
Related structures: EMD-35511

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-273355 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 356 amino acids
Theoretical weight: 39.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Cannabinoid receptor 1 Chain: R
Molecule details ›
Chain: R
Length: 310 amino acids
Theoretical weight: 35.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21554 (Residues: 99-408; Coverage: 66%)
Gene names: CNR, CNR1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
scFV16 Chain: S
Molecule details ›
Chain: S
Length: 266 amino acids
Theoretical weight: 28.64 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand Q2B 1 x Q2B
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-35511
Expression system: Spodoptera frugiperda

Quick links

Citations

1 citations in other articles

Differential Behavior of Conformational Dynamics in Active and Inactive States of Cannabinoid Receptor 1.
Isu et al. (2024)