8il5

X-ray diffraction
2.5Å resolution

Crystal structure of alcohol oxidase PcAOX(M59V/Q60P/R61N)(Phanerochaete chrysosporium)

Released:
Source organism: Phanerodontia chrysosporium
Entry authors: Wu B, Wang Y

Function and Biology Details

Reaction catalysed:
A primary alcohol + O(2) = an aldehyde + H(2)O(2)
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-269688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 657 amino acids
Theoretical weight: 73.32 KDa
Source organism: Phanerodontia chrysosporium
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A977TIR6 (Residues: 1-653; Coverage: 100%)
Gene name: AOX
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P4212
Unit cell:
a: 161.26Å b: 161.26Å c: 113.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.213
Expression system: Escherichia coli