› 8ilm

Electron Microscopy
3.3Å resolution

The cryo-EM structure of eight Rubisco large subunits (RbcL), two Arabidopsis thaliana Rubisco accumulation factors 1 (AtRaf1), and seven Arabidopsis thaliana Bundle Sheath Defective 2 (AtBSD2)

Released:
DOI: 10.2210/pdb8ilm/pdb
PDB entry 8ilm coloured by chain, front view.
PDB entry 8ilm coloured by chain, side view.
PDB entry 8ilm coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into the functions of Raf1 and Bsd2 in hexadecameric Rubisco assembly.
Wang R, Song H, Zhang W, Wang N, Zhang S, Shao R, Liu C
Mol Plant (2023)
PMID: 37853692
Related structures: EMD-35536

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonadecamer (preferred)
PDBe Complex ID:
PDB-CPX-233882 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ribulose bisphosphate carboxylase large chain Chains: A, B, F, G, H, I, J, K
Molecule details ›
Chains: A, B, F, G, H, I, J, K
Length: 472 amino acids
Theoretical weight: 52.52 KDa
Source organism: Synechococcus elongatus PCC 6301
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00880 (Residues: 1-472; Coverage: 100%)
Gene names: cbbL, rbcA, rbcL, syc0130_c
Sequence domains:
Protein BUNDLE SHEATH DEFECTIVE 2, chloroplastic Chains: C, L, M, N, O, P, Q
Molecule details ›
Chains: C, L, M, N, O, P, Q
Length: 81 amino acids
Theoretical weight: 8.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SN73 (Residues: 57-136; Coverage: 59%)
Gene names: At3g47650, BSD2, F1P2.200
Rubisco accumulation factor 1.2, chloroplastic Chains: D, E, R, S
Molecule details ›
Chains: D, E, R, S
Length: 389 amino acids
Theoretical weight: 43.73 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SR19 (Residues: 62-449; Coverage: 86%)
Gene names: At3g04550, F7O18.2, RAF1.2
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-35536
Expression system: Escherichia coli BL21(DE3)

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