› 8in2

X-ray diffraction
3.08Å resolution

4,5-DOPA-extradiol-dioxygenase from Beta vulgaris

Released:
DOI: 10.2210/pdb8in2/pdb
PDB entry 8in2 coloured by chain, front view.
PDB entry 8in2 coloured by chain, side view.
PDB entry 8in2 coloured by chain, top view.
Source organism: Beta vulgaris
Entry authors: Chiang CC, Hsu CH

Function and Biology Details

Reaction catalysed: 
L-dopa + O(2) = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4,5-DOPA dioxygenase extradiol Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 30.72 KDa
Source organism: Beta vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70FG7 (Residues: 1-268; Coverage: 100%)
Gene name: DODA
Sequence domains: Catalytic LigB subunit of aromatic ring-opening dioxygenase

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P3121
Unit cell:
a: 95.873Å b: 95.873Å c: 124.908Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.167 0.22
Expression system: Escherichia coli

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