› 8ioj

Electron Microscopy
4.1Å resolution

The Rubisco assembly intermidiate of Rubisco large subunit (RbcL) and Arabidopsis thaliana Rubisco accumulation factor 1 (AtRaf1)

Released:
DOI: 10.2210/pdb8ioj/pdb
PDB entry 8ioj coloured by chain, front view.
PDB entry 8ioj coloured by chain, side view.
PDB entry 8ioj coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into the functions of Raf1 and Bsd2 in hexadecameric Rubisco assembly.
Wang R, Song H, Zhang W, Wang N, Zhang S, Shao R, Liu C
Mol Plant (2023)
PMID: 37853692
Related structures: EMD-35620

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233884 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ribulose bisphosphate carboxylase large chain Chains: A, B, C, D, H, I, J, K
Molecule details ›
Chains: A, B, C, D, H, I, J, K
Length: 472 amino acids
Theoretical weight: 52.52 KDa
Source organism: Synechococcus elongatus PCC 6301
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00880 (Residues: 1-472; Coverage: 100%)
Gene names: cbbL, rbcA, rbcL, syc0130_c
Sequence domains:
Rubisco accumulation factor 1.2, chloroplastic Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 203 amino acids
Theoretical weight: 22.82 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SR19 (Residues: 62-264; Coverage: 45%)
Gene names: At3g04550, F7O18.2, RAF1.2
Sequence domains:
Rubisco accumulation factor 1.2, chloroplastic Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 151 amino acids
Theoretical weight: 16.64 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SR19 (Residues: 287-437; Coverage: 34%)
Gene names: At3g04550, F7O18.2, RAF1.2
Sequence domains: Rubisco Assembly chaperone C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-35620
Expression system: Escherichia coli BL21(DE3)

Quick links