› 8ip0

Electron Microscopy
3.6Å resolution

Cryo-EM structure of type I-B Cascade bound to a PAM-containing dsDNA target at 3.6 angstrom resolution

Released:
DOI: 10.2210/pdb8ip0/pdb
PDB entry 8ip0 coloured by chain, front view.
PDB entry 8ip0 coloured by chain, side view.
PDB entry 8ip0 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6714
Primary publication:
Structure and genome editing of type I-B CRISPR-Cas.
Lu M, Yu C, Zhang Y, Ju W, Ye Z, Hua C, Mao J, Hu C, Yang Z, Xiao Y
OpenAccess logo Nat Commun 15 4126 (2024)
PMID: 38750051
Related structures: EMD-35629

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-241680 (preferred)
Entry contents:
4 distinct polypeptide molecules
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
Type I-MYXAN CRISPR-associated protein Cmx8 Chain: J
Molecule details ›
Chain: J
Length: 551 amino acids
Theoretical weight: 63.7 KDa
Source organism: Synechocystis sp. PCC 6714
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A068N831 (Residues: 1-519; Coverage: 100%)
Gene name: D082_50500
Type I-MYXAN CRISPR-associated protein Cas5/Cmx5/DevS Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.59 KDa
Source organism: Synechocystis sp. PCC 6714
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A068N1Y0 (Residues: 1-237; Coverage: 100%)
Gene name: D082_50520
Fruiting body developmental protein R-like protein Chains: B, C, D, H, I, N, O
Molecule details ›
Chains: B, C, D, H, I, N, O
Length: 301 amino acids
Theoretical weight: 33.79 KDa
Source organism: Synechocystis sp. PCC 6714
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A068N458 (Residues: 1-301; Coverage: 100%)
Gene name: D082_50510
Type I-MYXAN CRISPR-associated protein Cmx8 Chains: K, L, M
Molecule details ›
Chains: K, L, M
Length: 124 amino acids
Theoretical weight: 14.54 KDa
Source organism: Synechocystis sp. PCC 6714
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A068N831 (Residues: 396-519; Coverage: 24%)
Gene name: D082_50500
DNA (5'-D(P*TP*CP*CP*AP*TP*GP*TP*TP*TP*AP*TP*CP*AP*CP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.49 KDa
DNA (41-MER) Chain: G
Molecule details ›
Chain: G
Length: 41 nucleotides
Theoretical weight: 12.73 KDa
DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
RNA (44-MER) Chain: F
Molecule details ›
Chain: F
Length: 44 nucleotides
Theoretical weight: 13.98 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-35629
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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