› 8iqd

X-ray diffraction
2.39Å resolution

Crystal structure of AsfvPrimPol N-terminal Prim/Pol domain in complex with Mn2+ and dCTP

Released:
DOI: 10.2210/pdb8iqd/pdb
PDB entry 8iqd coloured by chain, front view.
PDB entry 8iqd coloured by chain, side view.
PDB entry 8iqd coloured by chain, top view.
Source organism: African swine fever virus BA71V
Primary publication:
Structures and implications of the C962R protein of African swine fever virus.
Shao Z, Su S, Yang J, Zhang W, Gao Y, Zhao X, Zhang Y, Shao Q, Cao C, Li H, Liu H, Zhang J, Lin J, Ma J, Gan J
OpenAccess logo Nucleic Acids Res 51 9475-9490 (2023)
PMID: 37587714

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative primase C962R Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 254 amino acids
Theoretical weight: 28.37 KDa
Source organism: African swine fever virus BA71V
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0C5B022 (Residues: 21-272; Coverage: 26%)
Gene names: BA71V-C962R, C962R

Ligands and Environments

2 bound ligands:
Ligand MN 11 x MN
Ligand DCP 4 x DCP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL10U2
Spacegroup: C2
Unit cell:
a: 209.16Å b: 42.814Å c: 128.214Å
α: 90° β: 125.04° γ: 90°
R-values:
R R work R free
0.244 0.241 0.283
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations.
Petrini et al. (2023)