8iss

Electron Microscopy
3.19Å resolution

Cryo-EM structure of wild-type human tRNA Splicing Endonuclease Complex bound to pre-tRNA-ARG at 3.19 A resolution

Released:
Source organism: Homo sapiens
Related structures: EMD-35694

Function and Biology Details

Reaction catalysed:
PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-250365 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
tRNA-splicing endonuclease subunit Sen15 Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 19.76 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8WW01 (Residues: 1-171; Coverage: 100%)
Gene names: C1orf19, SEN15, TSEN15
Sequence domains: Sen15 protein
tRNA-splicing endonuclease subunit Sen2 Chain: B
Molecule details ›
Chain: B
Length: 465 amino acids
Theoretical weight: 53.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8NCE0 (Residues: 1-465; Coverage: 100%)
Gene names: SEN2, TSEN2
Sequence domains:
tRNA-splicing endonuclease subunit Sen34 Chain: C
tRNA-splicing endonuclease subunit Sen54 Chain: D
RNA (88-MER) Chain: E

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.19Å
Relevant EMDB volumes: EMD-35694
Expression system: Trichoplusia ni