› 8itf

Electron Microscopy
3.46Å resolution

Cryo-EM structure of the DMCHA-bound mTAAR9-Gs complex

Released:
DOI: 10.2210/pdb8itf/pdb
PDB entry 8itf coloured by chain, front view.
PDB entry 8itf coloured by chain, side view.
PDB entry 8itf coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of amine odorant perception by a mammal olfactory receptor.
Guo L, Cheng J, Lian S, Liu Q, Lu Y, Zheng Y, Zhu K, Zhang M, Kong Y, Zhang C, Rong N, Zhuang Y, Fang G, Jiang J, Zhang T, Han X, Liu Z, Xia M, Liu S, Zhang L, Liberles SD, Yu X, Xu Y, Yang F, Li Q, Sun JP
Nature (2023)
PMID: 37225986
Related structures: EMD-35705

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225741 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 42.01 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 377 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
Trace amine-associated receptor 9 Chain: R
Molecule details ›
Chain: R
Length: 348 amino acids
Theoretical weight: 38.93 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5QD04 (Residues: 1-348; Coverage: 100%)
Gene name: Taar9
Sequence domains: 7 transmembrane receptor (rhodopsin family)
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 285 amino acids
Theoretical weight: 30.36 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand 8IA 1 x 8IA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.46Å
Relevant EMDB volumes: EMD-35705
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

From odor to oncology: non-canonical odorant receptors in cancer.
Park et al. (2024)
1 more