8itl

Electron Microscopy
3.23Å resolution

Cryo-EM structure of GIPR splice variant 1 (SV1) in complex with Gs protein

Released:
Primary publication:
Molecular basis of signal transduction mediated by the human GIPR splice variants.
OpenAccess logo Proc Natl Acad Sci U S A 120 e2306145120 (2023)
PMID: 37792509
Related structures: EMD-35706

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-232422 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Gastric inhibitory polypeptide receptor Chain: R
Molecule details ›
Chain: R
Length: 364 amino acids
Theoretical weight: 42.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.73 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nanobody-35 Chain: N

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.23Å
Relevant EMDB volumes: EMD-35706
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli