› 8itl

Electron Microscopy
3.23Å resolution

Cryo-EM structure of GIPR splice variant 1 (SV1) in complex with Gs protein

Released:
DOI: 10.2210/pdb8itl/pdb
PDB entry 8itl coloured by chain, front view.
PDB entry 8itl coloured by chain, side view.
PDB entry 8itl coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of signal transduction mediated by the human GIPR splice variants.
Zhao F, Hang K, Zhou Q, Shao L, Li H, Li W, Lin S, Dai A, Cai X, Liu Y, Xu Y, Feng W, Yang D, Wang MW
OpenAccess logo Proc Natl Acad Sci U S A 120 e2306145120 (2023)
PMID: 37792509
Related structures: EMD-35706

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-528868 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Gastric inhibitory polypeptide receptor Chain: R
Molecule details ›
Chain: R
Length: 364 amino acids
Theoretical weight: 42.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P48546 (Residues: 22-421; Coverage: 82%)
  • Best match: P48546-3 (Residues: 22-385)
Gene name: GIPR
Sequence domains:
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.73 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04896 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.23 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.23Å
Relevant EMDB volumes: EMD-35706
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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