› 8izb

Electron Microscopy
3.06Å resolution

Lysophosphatidylserine receptor GPR174-Gs complex

Released:
DOI: 10.2210/pdb8izb/pdb
PDB entry 8izb coloured by chain, front view.
PDB entry 8izb coloured by chain, side view.
PDB entry 8izb coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for ligand recognition and signaling of the lysophosphatidylserine receptors GPR34 and GPR174.
Liu G, Li X, Wang Y, Zhang X, Gong W
OpenAccess logo PLoS Biol 21 e3002387 (2023)
PMID: 38048360
Related structures: EMD-35838

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-234042 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 29.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 62%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 376 amino acids
Theoretical weight: 40.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Probable G-protein coupled receptor 174 Chain: R
Molecule details ›
Chain: R
Length: 568 amino acids
Theoretical weight: 63.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BXC1 (Residues: 1-309; Coverage: 93%)
Gene names: FKSG79, GPCR17, GPR174
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

3 bound ligands:
Ligand UBL 1 x UBL
Ligand SER 1 x SER
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-35838
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Phosphatidylserine phospholipase A1 enables GPR34-dependent immune cell accumulation in the peritoneal cavity.
Tam et al. (2024)