› 8j1x

X-ray diffraction
2.25Å resolution

A near-infrared fluorescent protein of de novo backbone design

Released:
DOI: 10.2210/pdb8j1x/pdb
PDB entry 8j1x coloured by chain, front view.
PDB entry 8j1x coloured by chain, side view.
PDB entry 8j1x coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Using Protein Design and Directed Evolution to Monomerize a Bright Near-Infrared Fluorescent Protein.
Hu X, Xu Y, Yi J, Wang C, Zhu Z, Yue T, Zhang H, Wang X, Wu F, Xue L, Bai L, Liu H, Chen Q
ACS Synth Biol (2024)
PMID: 38552148

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
near-infrared fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 24.33 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2
Unit cell:
a: 64.33Å b: 50.545Å c: 66.651Å
α: 90° β: 112.379° γ: 90°
R-values:
R R work R free
0.197 0.196 0.214
Expression system: Not provided

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