› 8j3r

Electron Microscopy
2.95Å resolution

Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA

Released:
DOI: 10.2210/pdb8j3r/pdb
PDB entry 8j3r coloured by chain, front view.
PDB entry 8j3r coloured by chain, side view.
PDB entry 8j3r coloured by chain, top view.
Source organism: Acidibacillus sulfuroxidans
Entry authors: Hino T, Omura NS, Nakagawa R, Togashi T, Takeda NS, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro H, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Siksnys V, Ohmori T, Hoshino A, Nureki O
Related structures: EMD-35965

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229648 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endodeoxyribonuclease Cas12f1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 49.98 KDa
Source organism: Acidibacillus sulfuroxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2U3D0N8 (Residues: 1-422; Coverage: 100%)
Gene names: BM613_13600, cas12f1
Sequence domains: Putative transposase DNA-binding domain
DNA (37-MER) Chain: D
Molecule details ›
Chain: D
Length: 37 nucleotides
Theoretical weight: 11.39 KDa
Source organism: Acidibacillus sulfuroxidans
Expression system: Escherichia coli
DNA (38-MER) Chain: E
Molecule details ›
Chain: E
Length: 38 nucleotides
Theoretical weight: 11.7 KDa
Source organism: Acidibacillus sulfuroxidans
Expression system: Escherichia coli
RNA (118-MER) Chain: C
Molecule details ›
Chain: C
Length: 118 nucleotides
Theoretical weight: 38.29 KDa
Source organism: Acidibacillus sulfuroxidans
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.95Å
Relevant EMDB volumes: EMD-35965
Expression system: Escherichia coli

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