› 8j5p

Electron Microscopy
3.1Å resolution

Cryo-EM structure of native RC-LH complex from Roseiflexus castenholzii at 2,000lux

Released:
DOI: 10.2210/pdb8j5p/pdb
PDB entry 8j5p coloured by chain, front view.
PDB entry 8j5p coloured by chain, side view.
PDB entry 8j5p coloured by chain, top view.
Source organism: Roseiflexus castenholzii DSM 13941
Primary publication:
Carotenoid assembly regulates quinone diffusion and the Roseiflexus castenholzii reaction center-light harvesting complex architecture.
Xin J, Shi Y, Zhang X, Yuan X, Xin Y, He H, Shen J, Blankenship RE, Xu X
OpenAccess logo Elife 12 (2023)
PMID: 37737710
Related structures: EMD-35989

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
PDBe Complex ID:
PDB-CPX-240096 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Antenna complex alpha/beta subunit domain-containing protein Chains: 0, 2, 4, 6, 8, B, E, G, I, K, O, Q, S, U, W
Molecule details ›
Chains: 0, 2, 4, 6, 8, B, E, G, I, K, O, Q, S, U, W
Length: 55 amino acids
Theoretical weight: 6.43 KDa
Source organism: Roseiflexus castenholzii DSM 13941
UniProt:
  • Canonical: A7NQE9 (Residues: 1-55; Coverage: 100%)
Gene name: Rcas_3756
Sequence domains: Antenna complex alpha/beta subunit
Alpha subunit of light-harvesting 1 Chains: 1, 3, 5, 7, 9, A, D, F, H, J, N, P, R, T, V
Molecule details ›
Chains: 1, 3, 5, 7, 9, A, D, F, H, J, N, P, R, T, V
Length: 42 amino acids
Theoretical weight: 4.72 KDa
Source organism: Roseiflexus castenholzii DSM 13941
UniProt:
  • Canonical: Q83XD1 (Residues: 1-42; Coverage: 100%)
Gene name: pufA
MULTIHEME_CYTC DOMAIN-CONTAINING PROTEIN Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 34.92 KDa
Source organism: Roseiflexus castenholzii DSM 13941
UniProt:
  • Canonical: A7NQE7 (Residues: 1-320; Coverage: 100%)
Gene name: Rcas_3753
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 315 amino acids
Theoretical weight: 34.84 KDa
Source organism: Roseiflexus castenholzii DSM 13941
UniProt:
  • Canonical: A7NQE8 (Residues: 1-315; Coverage: 49%)
Gene name: Rcas_3754
Sequence domains: Photosynthetic reaction centre protein
Reaction center protein L chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 35.05 KDa
Source organism: Roseiflexus castenholzii DSM 13941
UniProt:
  • Canonical: A7NQE8 (Residues: 335-641; Coverage: 48%)
Gene name: Rcas_3754
Sequence domains: Photosynthetic reaction centre protein
Subunit X Chain: X
Molecule details ›
Chain: X
Length: 32 amino acids
Theoretical weight: 3.63 KDa
Source organism: Roseiflexus castenholzii DSM 13941
Subunit Y Chain: Y
Molecule details ›
Chain: Y
Length: 39 amino acids
Theoretical weight: 4.49 KDa
Source organism: Roseiflexus castenholzii DSM 13941
Subunit Z Chain: Z
Molecule details ›
Chain: Z
Length: 63 amino acids
Theoretical weight: 6.93 KDa
Source organism: Roseiflexus castenholzii DSM 13941

Ligands and Environments

7 bound ligands:
Ligand BCL 48 x BCL
Ligand KGD 30 x KGD
Ligand HEM 4 x HEM
Ligand BPB 3 x BPB
Ligand MQE 3 x MQE
Ligand PGV 3 x PGV
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-35989

Quick links